国际病理科学与临床杂志 2010, 30(1) 76- DOI:   10.3969/j.issn.16732588.2010.  ISSN: 1673-2588 CN: 43-1458/R
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间充质干细胞
心肌修复
凋亡
低氧
基因工程
预适应
本文作者相关文章
PubMed
低氧对MSC成心肌细胞中干细胞存活率的影响及对抗策略与原理
粘烨琦， 王婧谊综述罗庚求审校
中南大学基础医学院病理学系, 长沙 410078
摘要： 间充质干细胞（mesenchymal stem cells，MSCs）是一种具多向分化潜能的成体干细胞，具备修复心肌组织的能力。国内外学者做出了大量的努力，希望能够将干细胞移植治疗心脏疾病用于临床。但干细胞在移植后可发生大量的死亡，对治疗效果产生了重大影响。低氧是影响干细胞存活力较为重要的因素。低氧能够通过线粒体途径诱导MSC凋亡，使MSC的长期存活力下降。而抗凋亡途径（如磷脂酰肌醇3激酶/AKT信号通路、核因子κB途径)和抗凋亡蛋白（如Bcl2相关蛋白、热休克蛋白、低氧诱导蛋白1等）能够对抗低氧引起的细胞凋亡。利用这一原理，国内外学者采用了基因工程、预适应、药物联合治疗等策略以提高MSCs的长期存活率。
关键词： 间充质干细胞   心肌修复   凋亡   低氧   基因工程   预适应  
Effect of hypoxia on the survival rate of mesenchymal stem cells in myocardium and the mechanisms
NIAN Yeqi , WANG Jingyi, LUO Gengqiu
Department of Pathology, Xiangya School of Medicine, Central South University, Changsha 410078, China
Abstract: Mesenchymal stem cells （MSCs）, which are a type of adult stem cells with multiplex differentiation potential, have the ability to repair myocardium tissue. Great efforts had been made by the scholars to use stem cell transplantation for clinical treatment of cardiac disease. But after transplantation, large amounts of the stem cells are dead, which exerts negative effect on the therapy. Hypoxia is an important factor that affects the survival of stem cells. Through mitochondrial pathway, hypoxia can induce MSCs apoptosis to decrease its long term survival rate. Antiapoptotic pathways (like PI3kAkt and NFκB pathways) and antiapoptotic proteins (like bcl2 super family, heat shock protein and hypoxiainducible factor1) are able to counteract hypoxiainduced apoptosis. Based on these reports, experts from domestic and abroad have developed strategies such as genetic engineering, preconditioning and combination therapy to improve the long term survival of MSCs.
Keywords: mesenchymal stem cells;myocardial repair;apoptotic;hypoxia;gene engineering;preconditioning  
收稿日期 2009-12-23 修回日期 2010-01-23 网络版发布日期  
DOI: 10.3969/j.issn.16732588.2010.
基金项目:

通讯作者: 罗庚求
作者简介:
作者Email: luogengqiu@hotmail.com
参考文献：
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