国际病理科学与临床杂志 2010, 30(1) 71- DOI:   10.3969/j.issn.16732588.2010.  ISSN: 1673-2588 CN: 43-1458/R

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比较基因组杂交
肿瘤
软组织
肉瘤
本文作者相关文章
PubMed
软组织肿瘤的比较基因组杂交研究进展
蒙国照综述
桂林医学院病理学教研室/附属医院病理科, 广西 桂林 541001
摘要: 比较基因组杂交(comparative genomic hybridization,CGH) 是1992年建立起来的重大分子细胞遗传学分析技术,它在对整个基因组DNA拷贝数变异的检测方面是一种有效的方法。通过CGH检测,可找出染色体DNA拷贝数的变异特点,即基因拷贝数的扩增或丢失,从而确定相关的癌基因和抑癌基因所在的区域,为探讨肿瘤的发病机制提供依据。在过去的十几年中,用CGH对各种软组织肿瘤进行研究,已探测到了各种各样的有不同程度特异性的基因组的不平衡性,不仅开辟了探测各种癌症相关基因的新途径,并且找到了一些与临床相关的基因改变,可用于肿瘤的发生、发展、鉴别诊断、预后以及治疗等研究。
关键词 比较基因组杂交   肿瘤   软组织   肉瘤  
Progress of comparative genomic hybridization in soft tissue tumors
MENG Guozhao
Department of Pathology, School of Preclinical Medicine and Affiliated Hospital, Guilin Medical College, Guilin Guangxi 541001, China
Abstract: The advent of comparative genomic hybridization (CGH), which was established in 1992, had opened a reliable way for the detection of all genomic imbalances (copy number alterations of DNA) in each tumor (including archival material) without selection. With the CGH method, we can detect gene aberrations (gains or losses), then define the oncogene and the tumor suppressor gene, finally reveal the tumorigenesis of tumors. In the last decade, different patterns of chromosomal aberrations, including gains and losses of tumor DNA sequences, were detected in various soft tissue sarcomas by CGH. It not only provides a method to detect aberration genes, but also find a way to identify some gene aberrations related to clinicopathologic parameters. The CGH method was now extensively used in research of tumorigenesis, differential diagnosis, tumor development, tumor prognosis and treatment.
Keywords: comparative genomic hybridization;tumor;soft tissue;sarcoma  
收稿日期 2009-11-20 修回日期 2010-01-23 网络版发布日期  
DOI: 10.3969/j.issn.16732588.2010.
基金项目:

通讯作者: 蒙国照
作者简介:
作者Email: mengguozhao@yeah.net

参考文献:
[1]Szymanka J, Virolainen M, Tarkkanen M, et al. Overrepresentation of lq2123 and 12q1321 in lipomalike liposarcomas but not in benign lipomas:a comparative genomic hybridization study[J]. Cancer Genet Cytogenet,1997, 99 (1): 1418. [2]Suijkerbuijk RF, Olde Weghuis DE, Van den Berg M,et al. Comparative genomic hybridization as a tool to define two distinct chromosome 12derived amplification units in welldifferentiated liposarcomas[J]. Genes Chromosomes Cancer, 1994, 9(4):292295. [3]Fritz B, Schubert F, Wrobel G, et al. Microarraybased copy number and expression profiling in dedifferentiated and pleomorhic liposarcoma[J]. Cancer Res, 2002, 62(11): 29932998. [4]Pedeutour F, Forus A, Coindre JM, et al. Structure of the supernumerary ring and giant rod chromosomes in adipose tissue tumors[J]. Genes Chromosomes Cancer, 1999, 24(1):3041. [5]Pilotti S, Della Torre G, Lavarino C, et al. Molecular abnormalities in liposarcoma: role of MDM2 and CDK4containing amplicons at 12q1322[J]. J Pathol, 1998,185(2):188190. [6]Hostein I, Coindre JM, Derre J, et al. Comparative genomic hybridization study of paraffinembedded dedifferentiated liposarcoma fixed with Holland Bouin's fluid[J]. Diagn Mol Pathol, 2003, 12(3):166173. [7]Szymanska J, Tarkkanen M, Wiklund T, et al. Gains and losses of DNA sequences in liposarcomas evaluated by comparative genomic hybridization[J]. Genes Chromosomes Cancer, 1996,15(2):8994. [8]Weng WH, Wejde J, Ahlen J,et al. Characterization of large chromosome markers in a malignant fibrous histiocytoma by spectral karyotyping, comparative genomic hybridization (CGH), and array CGH[J]. Cancer Genet Cytogenet, 2004, 150(1):2732. [9]Ohguri T, Hisaoka M, Kawauchi S, et al. Cytogenetic analysis of myxoid liposarcoma and myxofibrosarcoma by arraybased comparative genomic hybridisation[J]. J Clin Pathol, 2006, 59(9):978983. [10]Tarkkanen M, Larramendy ML, Bohling T, et al. Malignant fibrous histiocytoma of bone: analysis of genomic imbalances by comparative genomic hybridisation and CMYC expression by immunohistochemistry[J]. Europ J Cancer, 2006, 42(8):11721180. [11]Idbaih A, Coindre JM, Derre J,et al. Myxoid malignant fibrous histiocytoma and pleomorphic liposarcoma share very similar genomic imbalances[J]. Lab Invest, 2005, 85(2):176181. [12]Dbaih A, Coindre JM, Derre J, et al. Myxoid malignant fibrous histiocytoma and pleomorphic liposarcoma share very similar genomic imbalances[J]. Lab Invest, 2005, 85(2):176181. [13]Larramendy ML, Gentile M, Soloneski S,et al. Does comparative genomic hybridization reveal distinct differences in DNA copy number sequence patterns between leiomyosarcoma and malignant fibrous histiocytoma?[J]. Cancer Genet Cytogenet, 2008,187(1):111. [14]Larramendy ML, Tarkkanen M, Blomqvist C, et al. Comparative genomic hybridization of malignant fibrous histiocytoma reveals a novel prognostic marker[J]. Am J Pathol, 1997, 151(4):11531161. [15]Weng WH, Ahlen J, Lui WO, et al. Gain of 17q in malignant fibrous histiocytoma is associated with a longer diseasefree survival and a low risk of developing distant metastasis[J]. Br J Cancer, 2003, 89(4):720726. [16]MezaZepeda LA, Kresse SH, BarraganPolania AH, et al. Array comparative genomic hybridization reveals distinct DNA copy number differences between gastrointestinal stromal tumors and leiomyosarcomas[J].Cancer Res, 2006, 66(18):89848993. [17]EI Rifai W, Sarlomo Rikala M, Knuutila S, et al. DNA copy number changes in development and progression in leiomyosarcomas of soft tissues[J]. Am J Pathol, 1998, 153(3):985990. [18]Parente F, Grosgeorge J,Coindre JM, et al. Comparative genomic hybridization reveals novel chromosome deletions in 90 primary soft tissue tumors[J]. Cancer Genet Cytogenet, 1999,115(2):8995. [19]Larramendy ML, Kaur S, Svarvar C, et al. Gene copy number profiling of softtissue leiomyosarcomas by arraycomparative genomic hybridization[J]. Cancer Genet Cytogenet, 2006, 169(2):94101. [20]Mittal KR, Chen F, Wei JJ,et al.Molecular and immunohistochemical evidence for the origin of uterine leiomyosarcomas from associated leiomyoma and symplastic leiomyomalike areas[J].Mod Pathol, 2009, 22(10):13031311. [21]Szymanska J,Serra M, Skytting B,et al. Genetic imbalances in 67 synovial sarcomas evaluated by comparative genomic hybridization[J]. Genes Chromosomes Cancer, 1998, 23(3):213219. [22]Nakagawa Y,Numoto K, Yoshida A, et al. Chromosomal and genetic imbalances in synovial sarcoma detected by conventional and microarray comparative genomic hybridization[J]. J Cancer Res Clin Oncol, 2006, 132(7):444450. [23]Skytting BT, Szymanska J, Aalto Y, et al. Clinical importance of genomic imbalances in synovial sarcoma evaluated by comparative genomic hybridization[J]. Cancer Genet Cytogenet, 1999,115 (1):3946. [24]Tarkkanen M, Kiuru Kuhlefelt S, Blonquvist C, et al. Clinical correlations of genetic changes by comparative genomic hybridization in Ewing sarcoma and related tumors[J]. Cancer Genet Cytogenet, 1999, 114 (1):3541. [25]Ferreira BI, Alonso J, Carrillo J,et al. Array CGH and geneexpression profiling reveals distinct genomic instability patterns associated with DNA repair and cellcycle checkpoint pathways in Ewing’s sarcoma[J].Oncogene, 2008, 27(14):20842090. [26]Maurici D, PerezAtayde A, Grier HE, et al. Frequency and implications of chromosome 8 and 12 gains in Ewing sarcoma[J]. Cancer Genet Cytogenet, 1998, 100 (2):106110. [27]Brisset S, Schleiermacher G, Peter M, et al. CGH analysis of secondary genetic changes in Ewing tumors: correlation with metastatic disease in a series of 43 cases[J]. Cancer Genet Cytogenet, 2001,130(1):5761. [28]Ozaki T, Paulussen M, Poremba C, et al. Genetic imbalances revealed by comparative genomic hybridization in Ewing tumors[J]. Genes Chromosomes Cancer, 2001, 32(2):164171. [29]Armengol G, Tarkkanen M, Virolainen M, et al. Recurrent gains of 1q, 8 and 12 in the Ewing family of tumours by comparative genomic hybridization[J]. Br J Cancer, 1997, 75(10):14031409. [30]Nakagawa Y, Yoshida A, Numoto K, et al. Chromosomal imbalances in malignant peripheral nerve sheath tumor detected by metaphase and microarray comparative genomic hybridization[J]. Oncol Rep, 2006, 15(2):297303. [31]KiuruKuhlefelt S, ElRifai W, SarlomoRikala M, et al. DNA copy number changes in alveolar soft part sarcoma: a comparative genomic hybridization study[J]. Mod Pathol, 1998, 11(3):227231. [32]Lee MW, Jee KJ, Han SS, et al. Comparative genomic hybridization in epithelioid sarcoma[J]. Br J Dermatol, 2004,151(5):10541059. [33]Lee MW, Jee KJ, Ro JY, et al. Proximaltype epithelioid sarcoma: case report and result of comparative genomic hybridization[J]. J Cutan Pathol, 2004, 31(1):6771. [34]Linn SC, West RB, Pollack JR, et al. Gene expression patterns and gene copy number changes in dermatofibrosarcoma protuberans[J]. Am J Pathol, 2003, 163(6):23832395. [35]Bovee JV, CletonJansen AM, Rosenberg C, et al. Molecular genetic characterization of both components of a dedifferentiated chondrosarcoma, with implications for its histogenesis[J]. J Pathol, 1999, 189(4):454462. [36]Larramendy ML, Tarkkanen M, Valle J, et al. Gains, losses and amplifications of DNA sequences evaluated by comparative genomic hybridization in chondrosarcomas[J]. Am J Pathol, 1997, 150 (2):685691. [37]Hameed M, Ulger C, Yasar D, et al. Genome profiling of chondrosarcoma using oligonucleotide arraybased comparative genomic hybridization[J].Cancer Genet Cytogenet, 2009, 192(2):5659. [38]Miettinen MM, ElRifai W, SarlomoRikala M, et al. Tumor sizerelated DNA copy number changes occur in solitary fibrous tumors but not in hemangiopericytomas[J]. Mod Pathol, 1997, 10(12):11941200. [39]Morawietz L, Kuhnen C, Katenkamp D, et al. Unusual sarcomatoid neoplasm of the lung suggesting a myofibrosarcoma[J]. Virchows Arch, 2005,447 (6):990995. [40]Meng GZ, Zhang HY, Zhang Z, et al. Myofibroblastic sarcoma vs nodular fasciitis: a comparative study of chromosomal imbalances[J].Am J Clin Pathol, 2009, 131(5):701709.
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