国际病理科学与临床杂志 2010, 30(1) 27- DOI:   10.3969/j.issn.16732588.2010.  ISSN: 1673-2588 CN: 43-1458/R
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子宫内膜异位症
GnRHⅡ蛋白
子宫内膜
免疫组织化学
本文作者相关文章
PubMed
GnRHⅡ蛋白在子宫内膜异位症患者中的表达及意义
邹颖， 黄凤英
中南大学湘雅二医院妇产科， 长沙 410011
摘要： 目的：检测GnRHⅡ蛋白在子宫内膜异位症患者异位子宫内膜、在位子宫内膜和正常子宫内膜中的表达情况，同时分析其表达是否与子宫内膜月经周期有关。方法：采用免疫组织化学SP法检测GnRHⅡ蛋白在异位内膜、在位内膜及正常子宫内膜组织中的表达情况，并分析和比较其表达是否有差异。结果：GnRHⅡ蛋白在子宫内膜异位症患者异位、在位子宫内膜及正常子宫内膜中均有表达，阳性表达定位于子宫内膜腺体及间质细胞的细胞质；GnRHⅡ蛋白在异位内膜、在位内膜及对照组正常内膜的表达依次增强，两两比较差异有统计学意义（P＜0.05）；GnRHⅡ蛋白在正常子宫内膜分泌期表达强于增生期（P＜0.05），且以分泌早中期最强，显著强于增生期和分泌晚期（P＜0.01），而异位组或在位组的分泌期与增生期比较，差异无统计学意义（P＞0.05）。结论：GnRHⅡ蛋白在子宫内膜异位症的发病中以及在人类月经生理方面可能起重要作用。
关键词： 子宫内膜异位症   GnRHⅡ蛋白   子宫内膜   免疫组织化学  
Expression of GnRHⅡ protein in patients with endometriosis and its significance
ZOU Ying， HUANG Fengying
Department of Gynecology and Obstetrics, Second Xiangya Hospital, Central South University, Changsha 410011, China
Abstract: Objective To examine expression of GnRHⅡ protein in patients with and withoutendometriosis and to determine its relation to the menstrual cycle.MethodsThe expression of GnRHⅡ protein was detected in the normal, ectopic, eutopic endometrial tissues in women with and withoutendometriosis via immunohistochemistry SP method. ResultsGnRHⅡ was located in the cytoplasm of endometrial stromal cells and gland cells in all specimens. The expression of GnRHⅡ was lower in the ectopic endometrium than that in eutopic endometrium of patients with endometriosis （P＜0.05）, and it was lower in the eutopic and ectopic endometrium of patients with endometriosis than that in the endometrium of women without endometriosis (P＜0.05).The expression of GnRHⅡ was obviously higher in the early and middle secretory phase than that in the hyperplasia phase in normal endometrium (P＜0.05). However, there was no significant difference between the eutopic and ectopic endometrium in patients with endometriosis in the menstrual cycle (P＞0.05).Conclusion The expression of GnRHⅡ in the ectopic and eutopic endometrium may play a crucial role in the pathogenesis of endometriosis and human menstrual physiology.
Keywords: endometriosis；GnRHⅡ；endometrium；immuneohistochemistry   
收稿日期 2009-09-23 修回日期 2010-01-18 网络版发布日期  
DOI: 10.3969/j.issn.16732588.2010.
基金项目:

通讯作者: 黄凤英
作者简介:
作者Email: hfy6697@163.com
参考文献：
［1］Millar RP.GnRHⅡ and type Ⅱ GnRH receptors［J］. Trends Endocrinol Metab，2003，14（1）：3543. ［2］丰有吉, 沈铿. 妇产科学［M］. 北京：人民卫生出版社, 2008, 357365. FENG Youji, SHEN Keng. Gynecology and Obstetrics［M］. Beijing: People’s Medical Publishing House, 2008:357365. ［3］Olive DL.Gonadotropinreleasing hormone agonists for endometriosis［J］. N Engl J Med,2008,359(11):11361142. ［4］邓珊,郞景和,冷金花,等.药物治疗对子宫内膜异位症患者在位内膜凋亡的影响［J］.中国医学科学院学报，2007,29(2):252261. DENG Shan, LANG Jinghe, LENG Jinghua, et al. Effects of medical treatment on apoptosis in eutopic endometrium of patients with endometriosis［J］. Acta Academiae Medicinae Sinicae, 2007,29(2):252261. ［5］Enomoto M，Endo D，Kawashima S，et a1.Human Type Ⅱ GnRH receptor mediates effects of GnRH on cell proliferation［J］.Zool Sci，2004,21(7)：763770. ［6］Grundker C, Gunthert AR, Millar RP,et al. Expression of gonadotropinreleasing hormone II (GnRHⅡ) receptor in human endometrial and ovarian cancer cells and effects of GnRHII on tumor cell proliferation［J］.J Clin Endocrinol Metab,2002,87(3):14271430. ［7］Noyes RW,Hertig AT,Rock J.Dating the endometrial biopsy［J］.Fertil Steril,1950,1(1):325. ［8］zkara SK, Corakci A. Significantly decreased P27 expression in endometrial carcinoma compared to complex hyperplasia with atypia(correlation with p53 expression)［J］. Pathol Oncol Res, 2004, 10（2）:8997. ［9］Morimoto C, Osuga Y, Yano T, et al. GnRH II as a possible cytostatic regulator in the development of endometriosis［J］. Hum Reprod, 2005, 20(11): 32123218. ［10］韩利伟,姜卫国.GnRHa对子宫内膜异位症内膜间质细胞生长增殖及血管形成的影响［J］.现代妇产科进展,2008, 17(10):737739. HAN Liwei, JIANG Weiguo. GnRHa for endometriosis stromal cells proliferation and angiogenesis ［J］. Progress in Obstetrics and Gynecology, 2008, 17 (10) :737739. ［11］Harrison GS,Wierman ME,Nett TM,et al.Gonadotropinreleasing hormone and its receptor in normal and malignant cells［J］.Endocr Relat Cancer,2004,11(4):725748. ［12］Meresman GF, Bilotas M, Buquet RA, et al. Gonadotrop in releasing hormone agonist induces apoptosis and reduces cell proliferation in eutopic endometrial cultures from women with endometriosis［J］.Fertil Steril, 2003, 80(Suppl2):702  707. ［13］郎景和.子宫内膜异位症研究的新里程［J］.中华妇产科杂志, 2005,40(1): 34. LANG Jinghe. The new milestone of endometriosis study ［J］. Chinese Journal of Obstetrics and Gynecology, 2005,40(1):34. ［14］Raga F, Casan EM, Krussel JS,et al. Quantitative gonadotropinreleasing hormone gene expression and immunohistochemical localization in human endometrium throughout the menstrual cycle［J］.Biol Reprod,1998,59(3):661669.
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