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国际病理科学与临床杂志 2010, 30(1) 14- DOI: 10.3969/j.issn.16732588.2010. ISSN: 1673-2588 CN: 43-1458/R

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	槟榔碱
	2型糖尿病
	β细胞
	胰腺十二指肠同源盒因子1
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	PubMed

槟榔碱对2型糖尿病大鼠胰腺β细胞PDX1 mRNA表达的影响

亓竹青1，2，姚起鑫1，2，王光1，张伟1，周寿红1，凌红艳1，胡弼1

南华大学1.医学院生理学教研室； 2.药物药理研究所，湖南衡阳 421001

摘要：目的：探讨槟榔碱对2型糖尿病大鼠β细胞分泌功能及胰腺十二指肠同源盒因子1（pancreasduodenum homeobox1，PDX1）mRNA表达的影响。方法：采用高果糖饲料饲养Wistar大鼠12周制备2型糖尿病大鼠模型，40只实验动物随机分为5组：对照组、模型组、模型+1 mg/kg槟榔碱组、模型+5 mg/kg槟榔碱组、模型+10 mg/kg槟榔碱组。药物注射4周后股动脉取血检测空腹血糖、三酰甘油、总胆固醇、高密度脂蛋白、低密度脂蛋白、血清胰岛素，处死大鼠取胰腺组织，石蜡切片HE染色观察组织形态学变化，RTPCR检测β细胞内PDX1及胰岛素mRNA的表达水平。结果：（1）高糖作用引起Wistar大鼠胰腺β细胞PDX1 及胰岛素mRNA 的表达水平显著下降（P＜0.01）；（2）1 mg/kg和5 mg/kg槟榔碱处理能显著上调高糖喂养Wistar大鼠胰腺β细胞PDX1与胰岛素mRNA表达水平（P＜0.05，P＜0.01）。结论：槟榔碱可以通过上调PDX1和胰岛素基因表达,改善高糖环境下的β细胞胰岛素合成和分泌功能的损伤。

关键词：槟榔碱 2型糖尿病 β细胞胰腺十二指肠同源盒因子1

Effect of arecoline on PDX1 mRNA expression in rats with Type 2 diabetes meilitus

QI Zhuqing1，2, YAO Qixin1，2, WANG Guang1, ZHANG Wei1, ZHOU Shouhong1, LING Hongyan1, HU Bi1

1. Department of Physiology, School of Medicine; 2. Institute of Pharmacy & Pharmacology, University of South China, Hengyang Hunan 421001, China

Abstract: Objective To investigate the effect of arecoline on insulin secretion and the mRNA expression of pancreasduodenum homeobox1 (PDX1) in β cells in rats with Type 2 diabetes meilitus.Methods Wistar rats were fed with highfructose diet for 12 weeks to induce Type 2 diabetes meilitus. Forty Wistar rats were randomly divided into 5 groups: a control group; a diabetic group; and a low (1 mg/kg), medium (5 mg/kg), or high (10 mg/kg) dose of arecoline groups. Fasting blood glucose (FBG), triglyeride (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), and fasting serum insulin (FSI) were measured after treatment with arecoline for 4 weeks. Pancreatic tissue was fixed in 10% formaldehyde, sliced and HE dyed. RTPCR was used to detect the mRNA expression of PDXl and insulin．Results(1) Insulin secretion and mRNA levels of PDX1 were significantly decreased in rats with Type 2 diabetes meilitus compared with the control group. (2) Compared with the diabetic group, insulin secretion and mRNA levels of PDX1 were significantly increased in the medium and high dose of arecoline groups. Conclusion Arecoline can prevent the dysfunction of β cells of pancreas induced by high fructose via upregulation of PDX1.

Keywords: arecoline;Type 2 diabetes meilitus;β cell;pancreasduodenum homeobox1

收稿日期 2009-11-19 修回日期 2010-01-08 网络版发布日期

DOI: 10.3969/j.issn.16732588.2010.

基金项目:

通讯作者: 胡弼

作者简介:

作者Email: hubishengli@yahoo.com

参考文献：

［1］Zhao L,Guo M,Matsuoka TA, et al .The islet beta cellenriched MafA activator is a key regulator of insulin gene transcription［J］.J Biol Chem，2005,280(12):1188711894. ［2］Jeon SM,Kim HS,Lee TG,et al.Lower absorption of cholesteryl oleate in rats supplemented with areca catechu extract［J］.Ann Nutr Metab,2000,44(4)：170176. ［3］张春江，吕飞杰，陶海腾.槟榔活性成分及其功能作用的研究进展［J］.中国食物与营养,2008，6(6)：5052. ZHANG Chunjiang,L Feijie,TAO Haiteng.Research progress of constituents and bioactivities of areca nut［J］.Food and Nutrition in China,2008,6(6)：5052. ［4］Green PWC,Simmonds MSJ, Blaney WM. Toxicity and behavioural effects of dietborne alkaloids on larvae of the black blowfly, Phormia regina［J］.Med Vet Entomol,2002,16(2)：157160. ［5］Reaven GM,Hwang IS,Ho H. Fructose induced insulin resistance and hypertension in rats［J］．Hypertension,1987,10(5):512516. ［6］李光伟,潘孝仁,Stephen L, 等.检测人群胰岛素敏感性的一项新指数［J］.中华内科杂志,1993,32(10):656660. LI Guangwei,PAN Xiaoren, Stephen L,et al. A new insulinsensitivity index for the populationbased study［J］.Chinese Journal of Internal Medicine,1993,32(10):656660. ［7］Melloul D,Marshak S, Cerasi E. Regulation of PDX1 gene expression［J］. Diabetes, 2002, 51 (11) : S320S325. ［8］Sun Y, Chen L, Hou XG. Differentiation of bone marrow derived mesenchymal stem cells from diabetic patients into insulin producing cells in vitro［J］. Chin Med, 2007, 120 (9): 771776. ［9］Shiraki N, Lai CJ, Hishikari Y, et al. TGF2β signaling potentiates differentiation of embryonic stem cells to Pdx1 expressing endodermal cells［J］. Gene Cells,2005, 10 (6) : 503516. ［10］Stoffers DA,Kieffer TJ,Hussain MA,et al.Insulinotropic glucagon like peptide 1 agonists stimulate expression of homeodomain protein PDX1 and increase islet size in mouse pancreas ［J］. Diabetes,2000, 49 (5) : 741748. ［11］董凌燕,刘超,张梅,等．葡萄糖对胰岛细胞胰腺十二指肠同源盒基因1表达的影响［J］.江苏医药,2007,33(4)：381382. DONG Lingyan,LIU Chao,ZHANG Mei,et al. Effect of glucose on PDX1 gene expression in isolated rat islet cells［J］. Jiangsu Medical Journal,2007,33(4)：381382. ［12］Mona A,Richard ETS.Noninsulin therapies for Type 2 diabetes［J］. Nat Rev Drug Discov, 2007,10(6)：777778. ［13］胡茂清,罗玉,杨雪,等. 甘精胰岛素联合二甲双胍治疗2 型糖尿病患者的有效性和安全性［J］. 华西医学,2009,24 (5):11441146. HU Maoqing,LUO Yu,YANG Xue,et al. The efficacy and safety of the combination of metformin with glargine in Type 2 diabetic subjects［J］.West China Medical Journal, 2009,24(5):11441146. ［14］张伟,周寿红,凌红艳,等. 槟榔碱抑制氧化型低密度脂蛋白诱导的小鼠巨噬细胞炎症因子表达及其机制［J］. 中国动脉硬化杂志, 2009,17(4):269272. ZHANG Wei,ZHOU Shouhong,LING Hongyan,et al. Arecoline repressed inflammation factor expression of macrophages stimulated by oxidized low density lipoprotein and its mechanism ［J］.Chinese Journal of Arteriosclerosis, 2009,17(4):269272.

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