目的：检测10号染色体缺失张力蛋白磷酸酶（phosphatase and tensin homologue deleted on chromosome ten，PTEN）蛋白在中耳胆脂瘤上皮中的表达，探讨其在中耳胆脂瘤上皮细胞存活机制中的作用。方法：应用免疫组织化学SP法检测30例中耳胆脂瘤组织标本与15例正常外耳道皮肤标本中PTEN蛋白的表达。结果：PTEN蛋白阳性表达主要定位于上皮细胞核。PTEN在中耳胆脂瘤上皮组织中阳性表达率为36.7%，明显低于正常外耳道皮肤组的93.3%（P<0.01）。结论：PTEN在中耳胆脂瘤上皮的异常表达可能在胆脂瘤的发生、发展过程中起着一定的作用。胆脂瘤上皮中PTEN蛋白的表达缺失可能参与了胆脂瘤上皮细胞过度增殖机制。

Objective To determine the expression of  phosphatase and tensin homologue deleted on chromosome ten (PTEN) protein in middle ear cholesteatoma epithelium and to explore its role in cellular survival mechanisms of cholesteatoma epithelial cells.  MethodsSP immunohistochemical assay was used to detect expression of PTEN protein in 30 samples of human middle ear cholesteatoma tissues and 15 normal skin specimens from the external ear canal. ResultsPTEN immunoreactivity was detected in the nuclei of epithelial cells. The positive percentage of PTEN protein expression in middle ear cholesteatoma epithelium was 36.7%(11/30).  It is significantly lower than that in normal skin specimens  from the external ear canal (93.3% or 14/15, P<0.01). Conclusion The abnormal expression of PTEN in middle ear cholesteatoma epithelium plays a role in the formation and development of cholesteatoma. Loss of PTEN function in cholesteatoma may be related to the overproliferation of cholesteatoma epithelial cells.

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