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国际病理 2007, 27(5) 1-375 DOI:
ISSN: 0412-1961 CN: 21-1139/TG |
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| 论著 |
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鼻咽癌转移相关的分泌蛋白质的筛选 |
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向亚莉1,2，易红1，李茂玉1，张鹏飞1，李 萃1，
彭芳1，阮林1，陈主初1，肖志强1 |
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1.中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室，长沙 410008； 2.深圳市宝安区沙井人民医院，深圳 518104 |
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摘要：
目的：筛选鼻咽癌（nasopharyngeal carcinoma, NPC）转移相关的分泌蛋白质，为阐明NPC转移机制以及筛选NPC转移分子标志物提供实验依据。方法：应用二维凝胶电泳（two-dimensional electrophoresis，2-DE）技术分离一对来自同一亲本，具有不同转移潜能的NPC细胞系5-8F和6-10B细胞的分泌蛋白质，图像分析识别差异表达的蛋白质点，基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)和电喷雾-四极杆-串联质谱(ESI-Q-TOF MS/MS)对差异表达的蛋白质点进行鉴定。Western印迹法检测差异分泌蛋白质nm23-H1在两株细胞中的表达水平。结果：建立了5-8F和6-10B分泌蛋白质的2-DE图谱，质谱分析鉴定出14个非冗余的分泌蛋白质，其中Oncoprotein18等蛋白质在高转移潜能NPC细胞系5-8F中的表达水平高于无转移潜能的NPC细胞系，而nm23-H1等蛋白质在5-8F细胞系中的表达水平低于6-10B细胞系。Western印迹分析证实了nm23-H1在5-8F和6-10B细胞分泌蛋白质中的差异表达水平。结论：所鉴定的14个非冗余的差异分泌蛋白质为研究NPC转移机制以及筛选NPC转移分子标志物提供了实验依据。 |
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关键词：
鼻咽癌
转移
分泌蛋白质
双向凝胶电泳
质谱
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Identification of metastasis-related secretory proteins in
human nasopharyngeal carcinoma cells |
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XIANG Ya-li1,2, YI Hong1, LI Mao-yu1, ZHANG Peng-fei1,
LI Cui1, PENG Fang1, RUAN Lin1, CHEN Zhu-chu1, XIAO Zhi-qiang1
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1.Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University,
Changsha 410008; 2.Shajing People’s Hospital, Shenzhen 518104, China
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Abstract:
ObjectiveTo screen metastasis-related secretory proteins in nasopharyngeal carcinoma (NPC), and provide experimental evidences for elucidating the metastatic mechanism of NPC and screening the metastatic biomarkers of NPC. MethodsThe secretory proteins of a pair of NPC cell lines 5-8F and 6-10B with different metastatic potential and the same genetic background were separated by two-dimensional gel electrophoresis(2-DE), respectively. PDQuest software was applied to analyze 2-DE images, the differentially expressed protein spots between 5-8F and 6-10B were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS) and electrospray ionization-quadrupole time-of-flight MS/MS (ESI-Q-TOF MS/MS). The expression levels of differential protein nm23-H1 in the secretory proteins of two cell lines were detected by Western blot. Results2-DE maps of secretory proteins from 5-8F and 6-10B were established. Fourteen non-redundant differential expression proteins were identified by mass spectrometry. Some differential proteins such as oncoprotein 18 were significantly higher in highly metastatic 5-8F than those in non-metastatic 6-10B, and other differential proteins such as nm23-H1 were significantly lower in highly metastatic 5-8F than that in non-metastatic 6-10B. The differential expression levels of nm23-H1 in secretory proteins of 5-8F and 6-10B were confirmed by Western blot. ConclusionFourteen non-redundant differential expression proteins will be valuable for studying the metastatic mechanism of NPC and screening the metastatic biomarkers of NPC. |
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Keywords:
nasopharyngeal carcinoma
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收稿日期 2007-06-19 修回日期 2007-07-27 网络版发布日期 |
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DOI: |
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基金项目:
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通讯作者: 肖志强 |
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作者简介: |
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作者Email: |
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