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国际病理 2007, 27(6) 3-480 DOI:
ISSN: 0412-1961 CN: 21-1139/TG |
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| 论著 |
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人核受体hLRH-1不同变异体在多种肿瘤组织和细胞系中的表达 |
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邓小军1，王水良1，兰风华1，柯龙凤1，黎成金2，曾志勇3 |
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南京军区福州总医院1.全军检验医学研究所分子医学研究中心；
2.普通外科； 3.心胸外科，福州 350025 |
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摘要：
目的:初步分析人核受体hLRH-1不同变异体在多种肿瘤组织和细胞系中的表达。方法:常规RT-PCR法分析hLRH-1v1和hLRH-1在肝癌等多种肿瘤组织和HepG2等肿瘤细胞系中的表达情况，实时定量PCR法分析hLRH-1v1，hLRH-1和hOct4在HepG2，SMMC-7721和BEL-7402三株肝癌细胞系的表达水平。结果:hLRH-1v1的表达见于所有检测的12种类型肿瘤和8种恶性肿瘤细胞系，hLRH-1的表达则仅在12种类型肿瘤中的6种肿瘤组织中可检测到，但8种恶性肿瘤细胞系均为hLRH-1阳性表达；在HepG2，SMMC-7721和BEL-7402三株肝癌细胞系中hLRH-1v1和hOct4的表达呈正相关。结论:hLRH-1v1在肿瘤中广泛表达，可能在维持肿瘤干细胞的自我更新中发挥重要作用。 |
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关键词：
核受体hLRH-1
变异体
肿瘤
基因表达分析
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Expressions of different variants of human nuclear receptor
hLRH-1 in multiple cancer tissues and cell lines |
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DENG Xiao-jun1, WANG Shui-liang1, LAN Feng-hua1,
KE Long-feng1, LI Cheng-jin2, ZENG Zhi-yong3
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1.Center for Molecular Medicine, Institute of Laboratory Medicine; 2.Department of General Surgery;
3.Department of Cardiothoracic Surgery, Fuzhou General Hospital, Fuzhou 350025, China
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Abstract:
ObjectiveTo investigate the expression profiles of different isoforms of hLRH-1 in multiple cancer tissues and cell lines. MethodsRegular RT-PCR was carried out to analyze the expressions of hLRH-1v1 and hLRH-1 in multiple cancer tissues including hepatocellular carcinoma, and cancer cell lines such as HepG2. The relative mRNA abundance of hLRH-1v1, hLRH-1 and hOct4 in HepG2, SMMC-7721 and BEL-7402 was also analyzed by real time quantitative PCR. ResultsThe hLRH-1v1 was widely expressed in all detected 12 types of cancer tissues and 8 cancer cell lines, while hLRH-1 was expressed only in 6 out of 12 types of cancer tissues, although all 8 cancer cell lines were shown to have expression of hLRH-1. In HepG2, SMMC-7721 and BEL-7402, the expression level of hLRH-1v1 was correlated with that of hOct4. ConclusionhLRH-1v1 is generally expressed in tumors where it may play important role in self-renewal of cancer stem cell. |
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Keywords:
nuclear receptor hLRH-1
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收稿日期 2007-07-04 修回日期 2007-09-06 网络版发布日期 |
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DOI: |
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基金项目:
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通讯作者: 王水良 |
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