|
|
国际病理 2009, 29(4) 277-283 DOI:
ISSN: 0412-1961 CN: 21-1139/TG |
|
|
|
|
本期目录 |
下期目录 |
过刊浏览 |
高级检索
[打印本页]
[关闭]
|
|
| 论著 |
|
|
金纳多对内毒素诱导小鼠急性肺损伤保护作用机制的初步研究 |
|
|
郭争鸣1，陈懿2，刘惠君2，肖跃群1，宋海鹏1，王昭昭1，瞿晓琳1，罗自强2 |
|
|
1.湖南中医药高等专科学校基础部，湖南株洲 412012; 2.中南大学湘雅医学院生理学系，长沙 410078 |
|
|
摘要：
目的：初步探讨银杏叶提取物金纳多（Ginaton）对内毒素（lipopolysaccharide, LPS）诱导小鼠的急性肺损伤(acute lung injury, ALI)保护作用的可能机制。方法：于小鼠腹腔注射LPS（10 mg/kg）复制ALI动物模型。将小鼠随机分为对照组、LPS组、Ginaton组和Ginaton＋LPS组。观察各组肺组织病理学改变，测量肺湿/干重比，支气管肺泡灌洗液蛋白含量及乳酸脱氢酶（lactate dehydrogenase, LDH）活性，测量丙二醛（malondialdehyde, MDA）、一氧化氮合酶（nitric oxide synthase, iNOS）和髓过氧化物（myeloperoxidase,MPO)，免疫组织化学方法检测血红素加氧酶（heme oxygenase HO-1）及iNOS蛋白表达。结果： 金纳多可有效减轻LPS所致肺组织病理学变化，并降低肺湿/干重比和肺泡灌洗液中蛋白含量，降低肺泡灌洗液中LDH活性、肺组织MPO和iNOS活性，同时MDA和NO含量下降。免疫组织化学结果显示，LPS组iNOS表达上升（P＜0.01），而血红素加氧酶（HO-1）蛋白表达未见明显变化；而预先给予Ginaton可显著提高HO-1的表达，降低iNOS的表达（P＜0.01）。结论：Ginaton可减轻LPS所致急性肺组织损伤，其机制可能与诱导HO-1的表达，下调iNOS的表达和活性有关。 |
|
|
关键词：
银杏提取物
急性肺损伤
内毒素
血红素加氧酶
一氧化氮
|
|
|
Ginkgo biloba extract (Ginaton) attenuates acute lung
injury induced by lipopolysaccharide in mice |
|
|
GUO Zhengming1, CHEN Yi2, LIU Huijun2, XIAO Yuequn1, SONG Haipeng1,
WANG Zhaozhao1, QU Xiaolin1, LUO Ziqiang2
|
|
|
1.Department of Basic Science, Hunan Traditional Chinese Medicine College, Zhuzhou Hunan 412012;
2.Department of Physiology,Xiangya School of Medicine,Central South University, Changsha 410078, China
|
|
|
Abstract:
Objective To determine the protective effect of Ginkgo biloba extract (Ginaton) on acute lung injury induced by lipopolysaccharide (LPS) and its protective mechnism in mice. MethodsThe Kunming mice were randomly allocated into sham, LPS, Ginaton, and Ginaton＋LPS groups. LPS was intraperitoneally injected at a dose of 10 mg/kg body weight in the LPS group, along with equal volumes of saline in the sham group. Ginaton＋LPS group and Ginaton group were treated with Ginaton (100 mg/kg, intraperitoneally) 2 h prior to the injection of LPS or saline, respectively. The mice were killed 8 h after LPS challenge The bronchoalveolar lavage fluid (BALF) samples were analyzed for the concentration of total protein, lactate dehydrogenase (LDH) activity. The lung samples were taken for histologic evaluation and for determination of wet-to-dry (W/D) lung weight, malondialdehyde (MDA) level, myeloperoxidase (MPO) activity, nitric oxide (NO) level, and inducible nitric oxide synthase (iNOS) activity, as well as immunohistochemistry analysis for iNOS and heme oxygenase-1 (HO-1) protein expression. ResultsAll the indexes except HO-1 in the ginaton group were not significantly different to those in the control group. But lung tissue and BALF showed serious inflammatory changes in the LPS group. Compared with the LPS group, in addition to inflammatory reaction,the changes of protein content and LDH in BALF and W/D,level of MDA and NO, activity of MPO and iNOS in lung tissues were significantly reduced in the Ginaton+LPS group.(P＜0.05 or P＜0.01). The expression of iNOS protein in lung tissues was obvious higher in the LPS group than that of the Ginaton+LPS group (P＜0.01);however expression of HO-1 in the Ginoton + LPS group was significantly higher than that of the Ginoton group. Conclusion Ginaton can play a protective role against LPS-induced acute lung injury and it may be associated with the up-regulation of HO-1 and the down-regulation of iNOS expression and activity. |
|
|
Keywords:
Ginkgo biloba extract
|
|
|
收稿日期 2009-04-07 修回日期 2009-08-24 网络版发布日期 |
|
|
DOI: |
|
|
基金项目:
|
|
|
通讯作者: 罗自强 |
|
|
作者简介: |
|
作者Email: |
|
|
| 参考文献： |
|
| 本刊中的类似文章 |
|
| 文章评论 |
|
|
|
| Copyright by 国际病理 |
