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国际病理 2008, 28(1) 1-5 DOI:
ISSN: 0412-1961 CN: 21-1139/TG |
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| 论著 |
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Raf激酶抑制蛋白对鼻咽癌细胞增殖的影响 |
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欧阳果良，陈彦，易红，冯雪萍，张鹏飞，李茂玉，李萃，阮林，彭芳，陈主初，肖志强 |
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中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室，长沙 410008 |
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摘要：
目的：探讨Raf激酶抑制蛋白（RKIP）对鼻咽癌细胞增殖的影响。 方法：采用脂质体转染方法将反义RKIP核酸表达质粒pcDNA3.1(-)-asRKIP及空白载体pcDNA3.1(-)分别转染鼻咽癌细胞系6-10B细胞，用Western 印迹检测RKIP表达，建立RKIP表达下调的稳定转染细胞和对照细胞系。采用四甲基偶氮唑蓝（MTT）法、流式细胞术和软琼脂集落形成实验检测RKIP表达下调对鼻咽癌细胞增殖、细胞周期分布和停泊非依赖性生长的影响。结果：建立了RKIP表达下调的6-10B鼻咽癌细胞系；RKIP表达下调促进6-10B细胞增殖和停泊非依赖性生长，并加速其越过G0/G1期。结论：RKIP具有抑制鼻咽癌细胞增殖和停泊非依赖性生长的作用，可能在鼻咽癌发病中具有重要作用。 |
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关键词：
鼻咽癌
Raf激酶抑制蛋白
增殖
细胞周期
停泊非依赖性生长
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Effects of RKIP on the proliferation of nasopharyngeal carcinoma cells |
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OUYANG Guo-liang, CHEN Yan, YI Hong, FENG Xue-ping, ZHANG Peng-fei,
LI Mao-yu, LI Cui, RUAN Lin, PENG Fang, CHEN Zhu-chu, XIAO Zhi-qiang
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Key Laboratory of Cancer Proteomics of Ministry of Health of China, Xiangya Hospital, Central South University,
Changsha 410008, China
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Abstract:
ObjectiveTo investigate the effect of Raf kinase inhibitor protein (RKIP) on the proliferation of nasopharyngeal carcinoma (NPC) cells.MethodspcDNA3.1(-)-asRKIP plasmid that constitutively expressed antisense RKIP mRNA and empty plasmid pcDNA3.1(-) were transfected into human NPC cell line 6-10B cells using Lipofectamine2000 to establish cell line with RKIP down-regulation and control cell line, respectively. Methylthiazoletetrazolium (MTT) assay, flow cytometry (FCM) analysis and soft-agar colony formation assay were used to examine the effects of RKIP down-rgulation on proliferation, cell cycle distribution and anchorage independence growth of 6-10B cells, respectively.Results Stably transfected 6-10B cell line with RKIP down-regulation was successfully established. Down-regulation of RKIP in 6-10B cells could promote cell proliferation and anchorage independence growth, and increase the number of G0/G1 phase cells. ConclusionRKIP could inhibit cell proliferation and anchorage independence growth of human NPC cells, suggesting that RKIP might be involved in the pathogenesis of NPC. |
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Keywords:
nasopharyngeal carcinoma
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收稿日期 2007-04-05 修回日期 2007-05-11 网络版发布日期 |
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DOI: |
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基金项目:
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通讯作者: 肖志强 |
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作者简介: |
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